IRES

The PNL-BDNF-IRES 2-EGFP plasmid was identified to be correct.

构建的慢病毒载体质粒PNL -BDNF-IRES2-EGFP 经SalI和 BamHI 双酶切鉴定正确,三质粒共转染293T细胞 后荧光激发可见大量绿色荧光.

The Empire State Building does not sway.

纽约州建筑(EmpireStateBuilding)并不会摇摆.

Both genes were cloned into GFP expression vector pIRES 2 EGFP and transfected into HeLa cells.

将上述基因克隆入绿色荧光蛋白(GFP)表达载体pIRES2-EGFP中,转染人HeLa细胞,在荧光显微镜和电镜下观察转染细胞的形态和结构.

Youku gets more than 15 million unique visitors a day, or almost four million more than its main Chinese competitor, Tudou, according to iResearch.

据艾瑞咨询集团（iResearch）的数据，优酷网每天的独立访问量（unique visitor）超过1500万，较主要中国竞争对手土豆网高出近400万。

Unusual conditions such as refused takeoffs and.

localfires.中断起飞和火灾等突发情况.

Overall, setting an Expires header is abetter solution.

总的来说, 设置1个Expires头是更好的解决方案.