apoenzyme

Methods We extracted and purified cardiac cytoplasmic aspartate aminotransferase ( s-AST) apoenzyme, then immunized sheep to produce antibody against human s-AST. The activity of m-AST was determined with inhibition immunoassay, which can inhibit the activity of s-AST in the specimen.

方法提取纯化心肌细胞质型天门冬氨酸氨基转移酶同工酶（s-AST）酶蛋白，免疫羊制备抗人s-AST抗体，应用免疫抑制原理抑制标本中的s-AST活性，测定m-AST活性。

Light may affect its activity in two ways: by starting photosynthesis to produce glycolic acid, which induces the biosynthesis of the apoenzyme, and by accelerating the biosynthesis of flavines and thereby raising the level of FMN in tissues which increases the activity of the enzyme.

光也可以加速叶组织内FMN的合成，从而提高乙醇酸氧化酶的活性。

It also worth noting that the conformation of the Co(ⅱ)-substituted enzyme is similar to the native enzyme, and this may be the reason for restoring of the enzymatic activity of metal-free apoenzyme upon the addition of Co(ⅱ) ion.

脱辅基酶与Co（Ⅱ）离子结合，酶的主链构象恢复至与天然酶几近相同。可认为这是促使酶复活的内在因素。