The incubation solutions were taken on days 1,7,14,21 and 28, and centrifuged 10 000r/ min at 40C. The supernature solutions were analyzed for Ca2+ and hydroxyproline.

  • 分别于第1、7、14、21和28天取孵育液,10000r/min、40C离心,上清液作Ca2+测定和羟脯胺酸分析。

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