Methods To extract the rRNA of mycobacterium tuberculosis by guanidine thiocyanate splitting, phenol-chloroform extraction, isopropanol precipitating and proceeding dot-blotting with the probe emblematized by Digoxin, observing the results by enzyme presents color. Seventy-six clinical isolates were detected by this method.

  • 方法异硫氰酸胍裂解、酚-氯仿抽提、异丙醇沉淀法直接抽提结核分枝杆菌rRNA,地高辛标记探针斑点杂交,酶呈色观察结果检测结核分枝杆菌,用该法检测76例临床标本。

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