Optimized expression of a single-chain Fv antibody against human asialoglycoprotein receptor and determination of its affinity constant

Methods Affinity constant of the specific antibodies was determined with ELISA and capillary electrophoresis.

方法采用ELISA和毛细管电泳测定抗体抗原的亲和常数。

Measurement of Affinity Constant in Wastewater Bio-treatment

废水生物处理中基质亲和性常数的测定方法探讨

The affinity constant of soluble antibody was measured by non-competitive ELISA.

免疫细胞化学检测其抗原结合活性，非竞争ELISA法检测其亲和常数。

Measurement of affinity constant of monoclonal antibody by competitive antibody binding to antigen

以抗体竞争结合抗原测定单抗亲和力常数的研究

In the past, the Affinity Labeling and Site-Directed Mutagenesis are the primary methods to study amine receptor.

过去主要是通过亲和标记和点突变等实验方法进行研究。

The only thing special is the AFF logo in the middle.

唯一特殊的是中央“美国之家”的标志。

With AFF you can get a true image of the modern skydiving.

与财务司，您可以得到一个真正的形象，现代跳伞。

Some New Conclusions on the Aff in e Transformation in Plane Field

平面场仿射变换的若干新结论

Secondly the basic principle and the measurement model of AFF were developed.

其次建立了AFF系统的基本测量原理和数学模型；

The second AFF took place this time last year amid global economic turmoil.

去年的这个时候，第二届亚洲金融论坛在全球经济不景气的背景下召开。

" Fanny and Alexander," 1982, was one of Bergman's most prized productions during his lifetime, winning four Oscars, including the best foreign language film.

《芬尼与亚历山大》（1982）是伯格曼一生最受褒奖的作品之一，拿到了包括最佳外语片奖在内的四项奥斯卡奖。

Unsurprisingly, the name Alexa halved in a year, possibly due to potential confusion with Amazon's smart speaker.

不出所料，女孩名亚莉克莎（Alexa）的使用量在一年内减半，这可能是因为它与亚马逊智能音箱的语音助手同名，从而可能会发生混淆。

Social media users have also spoken about about their experiences with Alexa.

一次用户还阐述了使用Alexa的经历。

The Alexa service is the first Internet software product that learns from people.

在Alexa的服务是第一个互联网软件产品，学习的人。

A defeat in the second round marked the end of the line for last year's champion.

第二轮的失利表明去年的冠军得主已卫冕无望。

They've got to ride all the way to the end of the line

他们得一直坐到终点站。

First was September 11, which was hailed by some doomsters as the end of the line for unfettered globalisation, predicting that the ensuing security clampdown on the movement of people, goods and money would throw sand in the wheels of the global economy.

首先是9/11事件，一些末日论者认为，该事件标志着毫无节制的全球化进程已穷途末路，他们预言，随之而来的对人口、商品及资金流动实施的安全管制，会阻挠全球经济前进的步伐。

Since the establishment of the Qing dynasty, who had undergone the "Kang xi and Qianlong" boom, but intrusion in the latter part of the closed-door lock plus western capitalism, so the rule of the Qing dynasty inevitable to end of the line.

清朝自建立以来，曾经历了康乾盛世的繁荣发展时期，但是中后期的闭关锁国外加西方资本主义的入侵，使清王朝的统治不可避免的走向穷途末路。

There was silence at the other end of the line

电话那头没有声音。

There I approached a tiny vegetable stall with one Italian woman and her son selling a choice assortment of their produce-such as rich, almost algae-green leaves of spinach, tomatoes so red and bloody they looked like a cow's organs, and champagne-colored grapes with skins as tight as a showgirl's leotard.

我走近有个意大利妇女的小蔬菜摊，她和她儿子贩卖各式各样的产品——像是叶片丰润、绿藻色的菠菜，血红有如动物器官的番茄，外皮紧绷的香槟色葡萄。