Method: Apoptosis and intracellular free calcium concentration ( [ Ca 2+] I) were measured by flow cytometry, morphological changes and neuronal necrosis were observed with fluorescence microscope, and the lactic dehydrogenate ( LDH) release was measured by LDH kits.

  • 方法:流式细胞术检测细胞凋亡百分率及细胞内[Ca2+]i浓度,荧光显微镜观察细胞形态学变化和坏死百分率,用试剂盒显色法测定LDH的含量。

  • 互联网摘选 2025-01-20 13:32:32

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