And the optimum incubation time was 24h, the optimum temperature was 25 ° C, the optimum broth initial pH was 5.5. The optimum carbon source, nitrogen source and irritant factor were defined by single element, then optimized by CCRD.

  • 结果表明,最佳培养条件为25℃恒温厌氧培养24h且培养基初始pH值为5.5;通过单因素确定最佳碳源、氮源、刺激因子后采用三元二次回归正交旋转设计进行优化。

  • 互联网摘选 2025-01-20 17:21:04

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