Method: Human oral mucosa keratinocytes were isolated, cultured, stimulated with AANE, The supernatant was aspirated and the level of TNF α was detected using radioimmunoassay.

  • 方法:通过体外培养口腔黏膜角朊细胞,用不同浓度槟榔提取物对其进行干预,放射免疫方法检测不同干预时段培养上清中的肿瘤坏死因子α浓度。

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