Genome DNA was extracted by TKM method. Exon 10 of TSHR gene sequence was amplified, PCR production was detected by direct and reverse sequencing, and the results and human TSHR gene sequence Genbank were compared.

  • 用TKM法提取基因组DNA,PCR扩增TSHR基因第10外显子序列,对PCR产物正反向测序,测序结果同Genbank人TSHR基因序列对比。

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