[ Method] Use alternative reagent to establish modified alkaline lysis method for extraction of plasmid DNA and use agarose electrophoresis to examine the quality of plasmid DNA, ultraviolet spectrophotometry to evaluate its production and purity, restricted enzyme cutting to examine its availability.

  • 方法使用替代试剂,建立改良的碱裂解法进行质粒DNA提取,并用琼脂糖电泳验证所得的质粒的质量,紫外测量估算产量及纯度。限制性酶切验证其用度。

  • 互联网摘选 2025-02-14 14:12:54

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