Application value of FQ-PCR for detection of treponema pallidum DNA

Objective To evaluate an enzyme-linked immunosorbent assay ( ELISA) technique based on recombinant antigens for detection of antibodies against Treponema pallidum.

目的评价基因重组抗原ELISA法在梅毒螺旋体抗体检测中的意义。

The 4 rapid tests and TPHA were performed by using whole blood and serum respectively.

分别取全血和血清进行梅毒螺旋体血凝试验（TPHA）及4种试剂盒的检测。

Objective To approach double-primer PCR assay for simultaneous detection of Herpes simplex virus ⅱ( HSV-2) and Treponema pallidum.

目的建立双引物聚合酶链反应（PCR）用于同时快速检测梅毒螺旋体和单纯疱疹病毒Ⅱ型。

Conclusion The specific antibody of IgM treponema pallidum has high sensitivity and specificity, and hence can be applied in the diagnosis of syphilis in various periods.

结论梅毒螺旋体特异性IgM抗体检测灵敏度高、特异性强，适用于未经治疗的各个时期的梅毒诊断。

Clinical evaluation of specific IgM treponema pallidum antibody detection

梅毒螺旋体特异性IgM抗体检测的临床评价

An optimized method has been established to simultaneously detect multiple antibodies of HIV, TP and HCV in the serum by the way of microarray.

建立一种用蛋白质微阵列法可同时检测血清中艾滋病毒（HIV），丙型肝炎病毒（HCV）和梅毒螺旋体（TP）抗体的方法。

51 clinical samples were detected by dark-field microscopy and a double-primer PCR, the positive rates were 15.7% and 19.6%, respectively.

检测了51例临床标本，梅毒螺旋体暗视野显微镜检查阳性检出率为15.7%，双引物PCR阳性检出率为19.6%；

Diagnostic Significance of IgM Antibodies to Treponema Pallidum in Cerebrospinal Fluid for Neurosyphilis

梅毒螺旋体IgM抗体对于神经梅毒的诊断意义

Controlled experiments of testing anti-TP and anti-HIV by DIGFA and EIA

免疫胶体金与酶免疫检测HIV抗体和梅毒螺旋体抗体对照实验

Objective To evaluate the significance of IgM treponema pallidum specific antibody in the diagnosis of syphilis in various periods.

目的探讨梅毒螺旋体特异性IgM抗体检测在各个时期梅毒诊断中的意义。

Methods 2557 cases were detected by using ELISA with double antigen sandwich before blood transfusion.

方法：采用免疫ELISA法检测2557例输血前患者血清梅毒螺旋体抗体。

Objective: To establish PCR assay for rapid detection of Treponema pallidum.

目的：建立聚合酶链反应（PCR）用于快速检测梅毒螺旋体。

Conclusions In view of its high specificity and sensitivity, TP-IgM-WB could be used as a confirmatory test for the diagnosis of both symptomatic and asymptomatic neonatal congenital syphilis.

结论梅毒螺旋体IgM抗体蛋白印迹试验可作为一种确诊试验应用于新生儿胎传梅毒，尤其是胎传潜伏梅毒的早期诊断。

Methods: According to specific sequence of Treponema pallidum gene ( tpp47 gene), a pair of specific primers were designed and PCR assay was developed.

方法：根据梅毒螺旋体47ku膜蛋白基因序列，自行设计筛选一对特异寡核苷酸引物，建立梅毒螺旋体的PCR。

ELISA with Double Antigen Sandwich for Screening Speeific Serum Anti-TP Antibody in Blood Donors

应用双抗原夹心ELISA法筛查献血员梅毒螺旋体抗体

Construction of fusion bivalent DNA vaccine of Treponema pallidum and its immune activity

梅毒螺旋体融合双价DNA疫苗的构建及其免疫活性研究

Objective To understand the infection of TP among HIV carriers in Yunnan province, and to offer scientific bases for surveillance STD.

目的了解HIV感染者中梅毒螺旋体（TP）在我省的感染状况，为监测性传播疾病（STD）提供科学依据。

Objective To investigate the rates of false-positive reactions to Treponema Pallidum ( TP) of patients with malignant tumors and to analyze their clinical features.

目的了解恶性肿瘤患者梅毒螺旋体（Treponema pallidum，TP）抗体假阳性率并对阳性患者进行临床分析。

Amplification of the DNA Polymerase I Gene of Treponema Pallidum by PCR and its Application in Diagnosis of Primary Syphilis

PCR扩增梅毒螺旋体poIA基因及其在一期梅毒诊断中的应用

Passive particle agglutination test for detection of TP AB ( TPPA);

梅毒螺旋体特异性抗体明胶凝集试验（TPPA）；