The optimum number of ovaries in co culture is 20 Low temperature in ovary co culture can result in low callus rate and induce high green plant rate.

  • 低温子房共培养胚状体发生和愈伤率低,但绿苗分化率较高,施加PAA、谷氨酰胺对游离小孢子形成的胚状体有促进作用。

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