AIM: To explore the pathologic characteristics of immunologic injury in primary cultured rat hepatocytes, and to evaluate the protective effect of glycyrrhizin ( Grz) in vitro.
目的:探讨大鼠原代培养肝细胞免疫性损伤模型病理特征,评价甘利欣(Grz)护肝作用。
Aminoguanidine was used to confirm the target of Grz action. Expression of intercellular adhesion molecular-1 ( ICAM-1) was determined by immunocytochemistry. Percentage of apoptosis was detected with fluorescence microscope and flow cytometer.
免疫细胞化学法测ICAM-1表达,丫啶橙荧光染色法和流式细胞仪测肝细胞凋亡率,评价Grz护肝作用。
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