Cell lysates were homogenized in solubilization buffer supplemented with protease inhibitors on ice.
细胞裂解液在冰的蛋白抑制因子的增溶性缓冲液中匀浆.
D group: renal tumor cell lysates and TNF-α + IL-1� �.
D组: TNF - α+IL-1β+冻融抗原负载.
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