The time-lapse recording showed that at this stage the blastocoele collapsed at least once in25 of the26 embryos ( 96%).
实时监控录像显示在此阶段26枚胚胎中有25枚(96%)至少发生一次囊胚腔萎缩。
After thawing, 26 of the33 embryos ( most of which were composed of between two and ten cells) developed to blastocyst stage in which the blastocoele is formed.
解冻后,33枚胚胎中有26枚(大多数胚胎含有2到10个细胞)发育到囊胚阶段,此时囊胚腔已经形成。
Methods We isolated embryonic stem cells from inner cell mass of KM mice blastocysts. Then we transferred embryonic stem cells into blastocoele of C57BL/ 6J inbred mice by microinjection in order to construct chimeric mice.
方法从KM小鼠囊胚的内细胞团中分离培养胚胎干细胞,进行近交系C57BL6J小鼠受体囊胚腔内注射,进行嵌合体构建实验。
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