A method of determination of hemin was established through the catalytic oxidation reaction of hemin with H_2O_2.In this method, hemin was used as a mimetic peroxidase, 4-aminoantipyrine and phenol were used as hydrogen donator substrates.
以氯化血红素(Hemin)作为过氧化物模拟酶,4-氨基安替比林(4-aminoantipyrine,4-AAP)和苯酚(phenol)为氢供体底物,通过Hemin催化活化H2O2氧化底物偶联显色反应,建立了测定Hemin含量的分析方法。
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