pcr的例句大全

METHODS The traditional rat two kidney one clip ( 2K1C) renal hypertension model was modified and the expression of AT 1a mRNA, AT 1b mRNA was examined by quantitative reverse transcription polymerase chain reaction ( QRT PCR).

方法改进传统的两肾一夹肾性高血压大鼠实验模型制作方法，采用定量反转录聚合酶链式反应（QRT PCR）方法对AT1a mRNA、AT1b mRNA进行定量。

The expression of cardiac MLC-2 in 12 week-endurance trained rats was investigated by quantitative reverse transcription polymerize chain reaction technique ( QRT-PCR), using the 18S-RNA of rat as a internal refernce.

以大鼠18S－RNA为内参照，用定量反转录聚合酶链式反应（QRT－PCR）技术对对20只经过12周耐力训练的SD大鼠心肌中MLC－2基因的表达进行了研究。

Real-time PCR analysis indicated that the Prx showed different expression profiles in shrimp hemocytes and hepatopancreas after artificial infection with Vibrio anguillarum.

实时荧光定量PCR结果显示，弧菌感染后，该基因在对虾血细胞和肝胰脏中的转录水平都有明显变化并且表达模式不同。

Establishment and Application of Detection of Prune Dwarf Virus ( PDV) by RT-PCR

李矮缩病毒RT-PCR方法建立及检测应用

Semi-quantative RT-PCR was used to determine the effect of PrG on mRNA expression of COX-1 and COX-2 in RAW murine macrophage cell line stimulated by LPS.

半定量RT-PCR法检测PrG对LPS刺激的Raw小鼠巨噬细胞COX-1、COX-2mRNA表达的影响。

Two pair of primers containing attB adapter were designed separately by Gateway cloning technology. Platinum pfx DNA polymerase which can reduce the non-specific binding greatly was used during two PCR in which adding B sequence to the cloned gene.

根据Gateway克隆技术的要求，设计含有attB接头的引物，利用高保真的PlatinumpfxDNA聚合酶，通过PCR方法在克隆基因的两端加上B序列。

Establishment and application of PCR-SSP for detecting Mur blood group

Mur血型分子诊断方法的建立与应用

Study of RT-PCR methods for MHV ( mouse hepatitis virus) determination

小鼠肝炎病毒RT-PCR检测方法的研究

Methods: To obtain the sequence of NKM, we utilized 8 × glycin for hinge region to connect NIF with KGF by over-lap PCR.

方法：以8个甘氨酸作为铰链区，运用over-Lap PCR的方法将NIF与KGF连接，获得双重抗肺纤维化基因嵌合重组体NKM的基因序列。

Construction and Application of Methods of PCR and DNA Probe for MBV

斑节对虾杆状病毒（MBV）PCR和核酸探针检测方法的建立与应用

Objective To develop the modified molecular beacon-based dual fluorescent PCR assays for detection of SARS virus.

目的建立改良分子信标 - 双重实时荧光PCR检测SARS病毒 的方法,用于SARS的早期诊断和动物溯源.

Cloning and establishment of the semi-quantitative RT-PCR system of LHR partial gene of Goat's Uterus

山羊子宫中LHR基因片段的克隆及半定量RT-PCR检测方法的建立

Methods 40 JMG patients from South China were analyzed and HLA-DQA 1 alleles were determined using PCR-RFLP genotype method, compared with that of health controlled group.

方法采用PCR-RFLP方法，对40例少年型重症肌无力患儿的HLA-DQA1基因进行分型，并与健康对照组进行比较。

Some were embedded in paraffin, and the others were reserved at-80. ( 2) PCR was used to detect SV40, JCV and BKV in glioma and normal tissues.

所有标本一部分采用常规石蜡包埋，另一部分-80℃保存。（2）采用PCR方法检测SV40、JCV和BKV在肿瘤组织及正常脑组织中的病毒DNA；

On Pcr's Precision in Ora Formula The Content of Graph Theory and Its Development

论欧拉公式中P（cr）的精确度图论的发展

The mutation was confirmed by digestion of the PCR products with restriction enzyme.

发现突变后用特异性限制性内切酶对突变位点进行酶切，验证突变。

Analyses of PCR products in 82 HCV RNA positive Serra by restriction fragment length polymorphism

82例HCV RNA阳性血清聚合酶链反应产物的酶切分析

The genotypes of apoE, apoC-I and LRP genes were analyzed by using PCR and restriction fragment length polymorphism ( RFLP) techniques.

应用PCR-RFLP技术，对apoE、apoC-I与LRP基因进行分析。

The classification of integrons was performed by analyzing the positive PCR products using restriction fragment length polymorphism ( RFLP).

PCR-限制片段长度多态性（RFLP）筛查整合子并分类；

The construct was confirmed by PCR, restriction enzyme digestion and direct sequencing.

经限制性内切酶酶切和测序证实。