CCRD

Initial review study identified the central composite rotatable design ( CCRD) method as an appropriate method for efficient experiment design within the context of response surface calibration.

初步的预分析结果表明，中心复合旋转设计方法可以满足本项目的精度要求，而且具有相当高的工作效率，所以该试验设计方法被采用。

Based on the results of one-factor-at-a-time ( OFAT) experiment, central composite rotatable design ( CCRD) according to the principles of response surface methodology ( RSM) was carried out to optimize the enzymatic hydrolysis process.

在单因子实验的基础上，利用响应曲面法中的旋转中心组合设计对水解工艺进行了优化。

And the optimum incubation time was 24h, the optimum temperature was 25 ° C, the optimum broth initial pH was 5.5. The optimum carbon source, nitrogen source and irritant factor were defined by single element, then optimized by CCRD.

结果表明，最佳培养条件为25℃恒温厌氧培养24h且培养基初始pH值为5.5；通过单因素确定最佳碳源、氮源、刺激因子后采用三元二次回归正交旋转设计进行优化。

But there are many differences between the N-terminal and C-terminal CRDs ( NCRD and CCRD) of Gal-9, such as their sequences, oligosaccharide binding affinitie [ 5].

然而Gal-9的N端和C端结构域存在许多不同之处，如氨基酸序列、与寡聚糖的亲和力[5]等。