足够的,充分的;
Methods: The circled TA plasmid was digested and linearized with restriction endonuclease. The ends of the plasmid were repaired and made blunt with Klenow Fragment. A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase.
方法:将环化质粒以限制性内切酶消化成线性后,以Klenow大片段将末端补平,再用taq DNA聚合酶在线性载体的两个5′末端各加上一个非配对的T。
'That's fair enow,'said the old man Morgan.
“ 这话很公道.”老摩根说.
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