Methods: The circled TA plasmid was digested and linearized with restriction endonuclease. The ends of the plasmid were repaired and made blunt with Klenow Fragment. A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase.

Taq DNA polymerase is a kind of the highly heat-stable DNA polymerase, that was isolated from thermophilic bacterium Thermus aquaticus. Because of its high thermostability, high specificity, sensitivity and other excellent features, Taq DNA polymerase is widely used in the PCR amplification.

taq DNA聚合酶是一种来源于嗜热菌Thermus aquaticus的高度热稳定的DNA聚合酶，并以其耐高温、较高的特异性、灵敏度等优良特性被广泛应用在PCR扩增中。

With genome DNA as template, three upstream primers ( R1 、 R2 、 R3) targeting point mutation of K-ras oncogene codon 12 and common downstream primer ( R4) were added respectively. PCR was employed to amplify corresponding fragment with Taq DNA polymerase.

以基因组DNA为模板，分别加入针对K-ras第12密码子的三种主要突变方式设计的PCR上游引物R1、R2、R3和共同的下游引物R4，在taq DNA聚合酶作用下扩增相应片段。

Although the commercial production of Taq DNA polymerase has come true for many years and a lot of technique improvement has been made, the activity ratio of the enzyme preparation is not high, some methods of procedure too complicated, or the production cost is high.

虽然taq DNA聚合酶已经商品化生产多年，制备方法较多，但是目前一些方法生产的酶制剂比活性不高，而一些方法又过于繁琐，增加生产成本。

After Taq DNA polymerase had been isolated and purified from the thermophilic bacteria ( Thermus aquatics) in aquatic habitat by Randall K. Saiki and his colleagues in 1988, it was widely used in PCR techniques.

1988年，Randall K.Saiki等[1.2]从水生栖热菌（Thermus aquatics）中分离纯化到了taq DNA聚合酶，之后它广泛应用于PCR技术。

Concentration of primer, magnesium chloride, dNTP, template DNA, Taq DNA polymerase and annealing temperature in PCR and the quantity of PCR product and endonuclease and digesting time in digestion process affect the profiles of the whole experiment.

PCR反应体系中不同模板含量、引物浓度、taq DNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。

In order to establish the optimized SSR detection system, the concentrations of DNA, Mg~ ( 2+), Taq DNA polymerase, dNTPs, and the annealing temperature were optimized for SSR-PCR reaction.

为建立适宜的SSR反应体系，对影响SSR-PCR扩增的模板DNA浓度、Mg~（2+）的用量、taq DNA聚合酶浓度、dNTPs浓度、退火温度等因素进行了优化。

The DNA repair techniques with Taq DNA polymerase can greatly enhance the success rate of STR genotyping for high degrade samples, which is suitable for the STR genotyping of high degrade samples. 3.

使用taq DNA聚合酶修复技术可以极大地提高高度降解检材STR分型的成功率，适合高度降解检材STR分型的应用。

Methods Slides of metaphase chromosome were examined by the method of PCR in situ. And PCR reactions without Taq DNA polymerase, primer and bio 11 dUTP were set up as control groups.

方法应用原位PCR的方法对豫医无毛小鼠中期染色体标本的无毛基因进行检测，并设立PCR反应液中无taq DNA聚合酶、无引物、无bio-11-dUTP等进行多组对照。

[ METHOD] Different levels on concentration of DNA template, primer, dNTP mixture and Taq DNA polymerase and annealing temperature were set in this experimentation. All factor influence for SRAP-PCR in peanut genome was investigated.

【方法】对模板DNA、引物、dNTP mixture、taq DNA聚合酶浓度及退火温度设置不同梯度，研究各因素对PCR结果的影响；

One of the mechanisms causing gene mutation might be to change of the Taq DNA polymerase conformation. It interfers with the enzyme-substrate-dNTP binding site and further causes replication mismatch.

这一实验结果也说明了稀土是一种诱变剂，引起基因突变的机制之一可能是改变taq DNA聚合酶的立体构象，干扰了酶与底物dNTP结合位点，从而引起复制错配。

The total DNAs from 3 species of plants and 2 species of animals were amplified by RAPD ( random amplified polymorphic DNA) using 10 RAPD 10 nt primers and 3 different commercial Taq DNA polymerases, respectively.

随机选用了10条RAPD引物，采用3种商品taq DNA聚合酶体系，以5个不同物种的动植物总DNA为模板，进行RAPD扩增。

High content of protein, phenol, tannin, pigment and polysaccharide in plant tissues usually affect the activity of Taq DNA polymerase and Lead to failure of PCR reaction.

植物体内所含的蛋白质、单宁、酚类、多糖及色素等次生代谢物质影响taq DNA聚合酶的活性，是导致PCR扩增反应失败的主要因素。

Moreover, the concentrations of Mg2+, dNTP and Taq DNA polymerase in allele-specific PCR were higher than that in conventional PCR.

在等位基因特异PCR中，Mg2+、dNTP及taq DNA聚合酶的用量均大于普通PCR。

Taq DNA polymerase is an ideal enzyme for DNA sequencing because it's fast, highly processive and active over a broad range of temperature.

taq DNA聚合酶具有反应速度快、温度作用范围广及良好的续进性等特点，可视为一种理想的DNA顺序分析酶。

This study concentrated on extracting and purification of Taq and Pfu DNA polymerase in the recombinant strains.

本文主要研究从重组工程菌中分别提取Pfu DNA聚合酶及taq DNA聚合酶。

Optimization of Preparation Techniques of Taq DNA Polymerase

taq DNA聚合酶制备技术的优化

Study on the Functional Domain of Taq DNA Polymerase

taq DNA聚合酶功能区域的定位

Optimization of Recombinant Taq DNA Polymerase Expression in E.coli

重组taq DNA聚合酶在大肠杆菌中表达条件的优化

Direct Amplification of Target Sequences by Taq DNA Polymerise Using Double-stranded Rna as Templates

利用taq DNA聚合酶直接从双链RNA模板中扩增靶序列

General Taq DNA polymerase amplified long DNA fragments of Helicobacter pylori urease

普通taq DNA聚合酶扩增幽门螺杆菌尿素酶基因长片段