Methods Immunofluorescence staining was used to detect the location of protein kinase C ( PKC) in human oocyte ( GV, MI and M ⅱ stage), zygote, preimplantation embryo, and cortical granule ( CG) in human oocyte.

  • 方法对蛋白激酶C(PKC)在人卵母细胞、受精卵、种植前胚胎及皮质颗粒进行免疫荧光染色。

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