The Peyer's patches, mesenteric lymph node, intestinal mucosal epithelial tissues were prepared for the lymphocytes suspension and the changes of CD4+ and CD8+ T cells in these sites were determined by SABC immunohistochemistry;

  • 分离Peyer's patches(PP结)、肠系膜淋巴结(MLN)、小肠黏膜上皮制备淋巴细胞悬液并涂片,用免疫细胞化学法检测CD4~+、CD8~+T细胞亚群;

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