Besides, it introduces Peking University digital library portal implementation.

Objective To establish the specific 16S 23S rRNA gene spacer regions map of different bacteria by PCR, RFLP ( restriction fragment length polymorphism), DNA clone and sequences analysis.

目的建立检测不同菌种细菌的16S23S RRNA基因区间的特异图谱。

Methods HPV DNA was detected using polymerase chain reaction ( PCR) with a consensus primer and typed by using restriction fragment length polymorphism ( RFLP) method in 151 paraffin-embedded biopsy specimens from seven kinds of external genital epidermal proliferative lesions.

方法采用通用型引物PCR方法对151例外生殖器表皮增殖性病变进行HPV DNA的检测，应用限制性内切酶片段长度多态性（RFLP）方法进行HPV DNA分型。

The restriction fragment length polymorphism ( RFLP) of mitochondrial DNA ( mtDNA) of cobia was studied using 19 restriction endonucleases recognizing 5, 6 base pairs. The physical map of mtDNA has been constructed based on 6 endonucleases selected from 20 restriction endonucleases.

用19种识别5、6碱基的限制性内切酶对军曹鱼的mtDNA RFLP进行了分析研究，构建其mtDNA物理图谱；

RFLP ( restriction fragment length polymorphism) analysis showed that the transposon is 3 cM ( centiMorgan) away from the S locus and retains high transpositional activity with a germinal excision frequency of 20%.

限制性酶切片段长度多态性（RFLP）的分析证明该转座子距S位点约3cM（centiMorgan），并且保持了较高的转位活性，其配子切除频率高达20%。

The restriction fragment length polymorphism ( XbaI, EcoRI) of apolipoproteinB gene in relation to the atherothrombotic cerebrol infarction

动脉硬化性血栓性脑梗塞与载脂蛋白B基因XbaI、EcoRI多态的相关性研究

Molecular identification of Taenia in three regions of Yunnan and Guizhou provinces& restriction fragment length polymorphism ( rflp) analysis of the first internal transcribed spacer ( its1) of ribosomal DNA

云贵两省三地带绦虫的分子鉴定&核糖体DNA第一内转录间隔区（ITS1）限制性酶切片段长度多态性（RFLP）分析

For monitoring the dynamics of microbial community under simulated oil reservoir conditions, the effect of nutrient injection on the structure of oil reservoir microbial community was investigated by applying a cultivation and clone independent molecular technique& terminal restriction fragment length polymorphism ( T-RFLP).

为了监测油藏微生物群落动态，应用不依赖微生物培养与克隆的T-RFLP方法研究了模拟油藏环境中营养物的注入对油藏微生物群落结构的影响。

Objective Aim to set up the method of glycoprotein B genotype of human cytomegalovirus ( HCMV) analysis by using nested polymerase chain reaction ( nPCR) and restriction fragment length polymorphism ( RFLP).

目的建立人巨细胞病毒（HCMV）糖蛋白B（gB）基因的套式聚合酶链反应（nPCR）加限制性长度多态性分析（RFLP）的分型方法。

Effect of pentachlorophenol ( PCP) on COD and ammonium removal rates was investigated, and also changes of bacterial community within aerobic granular sludge were monitored by terminal restriction fragment length polymorphism ( T-RFLP) technique.

研究了五氯酚（PCP）对好氧颗粒污泥处理生活污水的影响，借助末端限制性酶切片段长度多态性（T-RFLP）技术考察了PCP存在时好氧颗粒污泥细菌组成的变化。

Methods Genetic information about Chinese Han X-ray worker obtained through questionnaire. Polymerase chain reaction and restriction fragment length polymorphism analysis were used to identify genotypes of every subject. Data analysis used SAS 6.12 statistical software.

方法问卷调查X射线工作者高血压病家族史，采用聚合酶链反应、限制性内切酶片断长度多态性的方法检测X射线工作者ACE和M235T基因多态性，SAS612软件进行资料分析。

The protection of domestic freshwater resources by the measures of trade restriction is restricted by the free trade system.

通过贸易限制措施保护国内水资源受到自由贸易体制的制约。

Therefore, to explore The Trade Associations restriction of competition law regulation from the perspective of the "anti-monopoly law" which theoretical and practical value are very obvious.

是故，从《反垄断法》角度探寻行业协会限制竞争行为的法律规制，其理论意义和实践价值皆十分明显。

Mr. Yu denied that the move constitutes a trade restriction.'

俞太尉否认这一举动构成了贸易限制。

However, once the subsidies go beyond the legal frame and turn into a disguise of trade restriction, it is necessary to regulate them.

但是，如果新能源补贴超过了一定限度，成为变相的限制贸易的手段，则需要对其进行合理的规制。

Anti-dumping is an effective trade restriction authorized by WTO. And it makes China's advantage of low labor cost become disadvantageous.

反倾销是被WTO认可的一种有效限制贸易的措施，它使中国的低劳动力成本优势成为劣势。

This article tries to, by analyzing the conflicts and harmonization between WTO law and such regulation, examine this creative unilateral trade restriction implemented on the basis of bilateral negotiations.

通过对该法规与WTO规则的冲突与协调进行的分析，可以看出在双边谈判的基础上实施的该法规是富有创造性的单边贸易限制措施。

The deterioration of global environments brings trade restriction measures into the consideration of environment protection and the prick up of international economy competition causes the cut down of tariff and non-tariff trade barrier.

全球环境问题的恶化使得人们开始考虑使用经贸限制措施促进环境的保护，国际经济竞争的加剧使关税和传统的非关税壁垒进一步削减。

Then discusses its economic effects: quantity control, price control and trade restriction of exported goods.

然后从绿色壁垒对进口产品的数量控制、价格控制和对贸易的制约三个方面对绿色壁垒的经济效应进行了分析。

Mainly makes a specific explanation on trade restriction effect and trade diversion effect.

这部分主要从贸易限制效应和贸易转移效应方面进行具体阐述。

Studies on the Active Groups of Pst I Restriction Endonuclease

限制性核酸内切酶PstⅠ活性基团的研究