Finally, alkaline lysis and anion exchange chromatography were used to release and purify plasmids.

The final yield of plasmid DNA from the broth after alkaline lysis is 56.61%, and its purity determined by agarose electrophoresis is almost 100%.

结果表明：以碱裂液中质粒DNA为计算基准的得率为56.61%、超螺旋质粒DNA的电泳纯度100%。

The procedure of Southern hybridization has been improved as follows: the plasmid DNA which contains DNA fragments for probe was isolated by alkaline lysis without utilizing cesium chloride gradient centrifugation.

对传统的Southern杂交法进行了改进：用碱变性和酚抽提法提取重组质粒，省去氯化铯梯度离心；

The plasmids were extracted by alkaline lysis technique.

应用碱裂解法提取相应菌株的质粒，对质粒谱进行分型。

Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafiltration with hollow fiber filter, hydrophobic chromatography and molecular sieve chromatography.

方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。

Use alkaline lysis method to extract the plasmid DNA. DNA constructs were identified by Pst I/ Bgl II digestion, denaturing polyacrylamide gel electrophoresis and DNA sequence analysis.

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

After such pre-treatments, the materials were denatured by a modified alkaline lysis procedure and their mtDNA was extracted.

经过处理后的材料分别再用改良的碱变性法[2～6]提取mtDNA，并比较相应处理的mtDNA。

Thirty genomic DNA of Fusarium were extracted with improved SDS alkaline lysis method and the ITS district of rDNA was especially amplified.

实验运用改良的SDS碱裂解法对30株镰刀菌基因组DNA进行了抽提，并对rDNA ITS区域进行了特异性扩增。

Objective to explore the action of main reagent alkaline lysis method of extracting plasmid DNA.

目的对碱裂解法提取质粒DNA中主要试剂的作用进行研究。

Methods Alkaline lysis assay was used to extract and purify the plasmid. Plasmid DNA was injected into tumor by microinjection assay.

方法：碱裂解法提取纯化质粒DNA，微量注射法直接将质粒DNA导入体内肿瘤；

The inhibin plasmid DNA was extracted and purified by alkaline lysis method and DEAE-fiber column chromatography and its concn.

采用碱裂解法与DEAE-纤维柱层析法提取与纯化抑制素质粒DNA，用分光光度法测定其浓度和纯度。

Using Alkaline Lysis Method for Recombine Plasmid DNA Extraction and PCR Verification

碱裂解法提取重组质粒DNA及PCR验证

METHODS the K-B manner and MIC are used to obtain drug-fast spectrum and the alkaline lysis is to extract small quantity of plasmid is got from cataphoresis of 0.7% agarose congelation.

方法耐药谱选用K&B法和MIC、Alkaline Lysis小量快速提取质粒，0.7%琼脂糖凝胶电泳获得质粒DNA图谱。

[ Method] Use alternative reagent to establish modified alkaline lysis method for extraction of plasmid DNA and use agarose electrophoresis to examine the quality of plasmid DNA, ultraviolet spectrophotometry to evaluate its production and purity, restricted enzyme cutting to examine its availability.

方法使用替代试剂，建立改良的碱裂解法进行质粒DNA提取，并用琼脂糖电泳验证所得的质粒的质量，紫外测量估算产量及纯度。限制性酶切验证其用度。

The plasmid DNA was extracted by Alkaline Lysis, and separated by electrophoresis on the gel.

Alkaline Lysis小量快速提取质粒DNA，凝胶电泳拍照；

In conclusion, sufficient DNA for PCR can be quickly extracted from transgenic rice food by alkaline lysis method. The stability of recombinant DNA in transgenic was different under different processing conditions.

由此可知，碱处理法能够快速、有效的提取转基因稻米制品中DNA，转基因稻米中的各种外源成分在不同加工条件下的稳定性不同。

An improved alkaline lysis adaptive to extracting plasmid DNA of Bacillus

一种适合芽孢杆菌质粒DNA提取的改良碱裂解法

Methods: Susceptibility to 7 antibiotic agents was tested by the disc diffusion method in 80 isolates, and plasmid DNA was obtained by alkaline lysis method in PPNG and TRNG strains.

方法：采用纸片扩散法检测80株淋病奈瑟菌对7种抗生素的敏感性；碱裂解法分析产青霉素酶的淋病奈瑟菌（PPNG）株及耐四环素的淋病奈瑟菌（TRNG）株的质粒图谱；

Samples were extracted with the 80% precooling and acidified methanol and conjugated abscisic acid in extracts were treated by alkaline lysis method.

样品以80%酸化预冷甲醇提取，使用碱裂解法分析检测ABA结合态。

Conclusion The alkaline lysis method is simple, rapid and efficient for extraction of serum HBV-DNA and suitable for HBV-DNA quantitative PCR detection.

结论碱裂解法提取HBV-DNA简单、快速，适用于HBV-DNA荧光定量测定。

New Application Technology of Chemical Nickel phosphorus Plating from alkaline bath at Middle Temperature

中温碱性化学镀镍磷实用新工艺研究