I stepped on the bus and looked down the aisle, all I saw were a bunch of people with bus looks minding their own business.

Do you think we'll ever get to meet an alien from outer space?

你觉得咱们会在外太空遇到外星人吗？

Alien from a single network rumor, the object we discussed in this paper is the network rumor which arises from emergency.

与单一网络谣言不同的是，突发事件中的网络谣言，是依附于突发事件这一载体而产生的。

I am an alien from another planet.

我是一位来自其他星球的外星人。

For the influence of various factors, American historiography after World War II tended to be alien from the grand narrative.

由于各种因素的影响，二战后的美国史学出现疏离宏大叙事的趋势。

Its duality makes drama translation alien from translations of other literary styles.

这一独特性决定了戏剧翻译与其他文体的翻译有所不同，对于翻译者来说，戏剧翻译更具挑战性。

This style is alien from genuine English.

这种文体与纯正的英语不同。

What's not good for my baby is sharing my home with a killer alien from outer space.

和外星杀手共处一室才是对宝宝不好

If you were an alien from Mars and sat in on one of these meetings, you would believe that a clients success or progress was not part of the thought process at all.

如果您是一位来自火星的外星人并且参与到其中的一个会议，你会感觉到，客户的成功和进步完全不是会议的议题。

It had an effect entirely alien from the one intended.

其结果兴所预期的完全相反。

Why not just tell people I'm an alien from Mars.

为什么不直接告诉人们我就是个来自火星的外星人呢？

Finally, alkaline lysis and anion exchange chromatography were used to release and purify plasmids.

最后用碱裂解法裂解细胞提取质粒，并将提取物进行阴离子交换层析以进一步纯化质粒。

The final yield of plasmid DNA from the broth after alkaline lysis is 56.61%, and its purity determined by agarose electrophoresis is almost 100%.

结果表明：以碱裂液中质粒DNA为计算基准的得率为56.61%、超螺旋质粒DNA的电泳纯度100%。

The procedure of Southern hybridization has been improved as follows: the plasmid DNA which contains DNA fragments for probe was isolated by alkaline lysis without utilizing cesium chloride gradient centrifugation.

对传统的Southern杂交法进行了改进：用碱变性和酚抽提法提取重组质粒，省去氯化铯梯度离心；

The plasmids were extracted by alkaline lysis technique.

应用碱裂解法提取相应菌株的质粒，对质粒谱进行分型。

Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafiltration with hollow fiber filter, hydrophobic chromatography and molecular sieve chromatography.

方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。

Use alkaline lysis method to extract the plasmid DNA. DNA constructs were identified by Pst I/ Bgl II digestion, denaturing polyacrylamide gel electrophoresis and DNA sequence analysis.

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

After such pre-treatments, the materials were denatured by a modified alkaline lysis procedure and their mtDNA was extracted.

经过处理后的材料分别再用改良的碱变性法[2～6]提取mtDNA，并比较相应处理的mtDNA。

Thirty genomic DNA of Fusarium were extracted with improved SDS alkaline lysis method and the ITS district of rDNA was especially amplified.

实验运用改良的SDS碱裂解法对30株镰刀菌基因组DNA进行了抽提，并对rDNA ITS区域进行了特异性扩增。

Objective to explore the action of main reagent alkaline lysis method of extracting plasmid DNA.

目的对碱裂解法提取质粒DNA中主要试剂的作用进行研究。

Methods Alkaline lysis assay was used to extract and purify the plasmid. Plasmid DNA was injected into tumor by microinjection assay.

方法：碱裂解法提取纯化质粒DNA，微量注射法直接将质粒DNA导入体内肿瘤；