ObjectiveTo assess the application value of fluorescence quantitative polymerase chain reaction for detection of treponema pallidum DNA.

Conclusions Treponema pallidum induced mainly the expression of CD11c~ (+) DC and initiated cell-mediated immune response.

结论梅毒螺旋体（TP）进入体内后主要诱导CD11c+型DC表达，引发Th1反应，启动细胞免疫；

Objective To find out the distribution of subtypes of T. pallidum ( TP) in Hengyang area and to provide laboratory basis for molecular epidemiological studies of TP infection.

目的了解衡阳地区梅毒螺旋体（TP）的分子亚型的分布，为梅毒螺旋体感染分子流行病学研究提供实验依据。

Objective To study the relationship between the different courses of Treponema pallidum ( TP) infection and the immunological changes of the host.

目的探讨梅毒螺旋体（TP）感染的不同病程与宿主免疫学变化的关系。

Diagnosis of Neonatal Congenital Syphilis with Treponema pallidum IgM Western Blot

梅毒螺旋体IgM抗体蛋白印迹试验诊断新生儿胎传梅毒的探讨

Furthermore, isolation and purification processes compromise the structural integrity of the fragile organisms.

另外，因梅毒螺旋体十分脆弱，分离和纯化过程可破坏Tp结构的完整性。

Application value of FQ-PCR for detection of treponema pallidum DNA

荧光PCR技术检测梅毒螺旋体DNA的意义

Objective To evaluate an enzyme-linked immunosorbent assay ( ELISA) technique based on recombinant antigens for detection of antibodies against Treponema pallidum.

目的评价基因重组抗原ELISA法在梅毒螺旋体抗体检测中的意义。

The 4 rapid tests and TPHA were performed by using whole blood and serum respectively.

分别取全血和血清进行梅毒螺旋体血凝试验（TPHA）及4种试剂盒的检测。

Objective To approach double-primer PCR assay for simultaneous detection of Herpes simplex virus ⅱ( HSV-2) and Treponema pallidum.

目的建立双引物聚合酶链反应（PCR）用于同时快速检测梅毒螺旋体和单纯疱疹病毒Ⅱ型。

Conclusion The specific antibody of IgM treponema pallidum has high sensitivity and specificity, and hence can be applied in the diagnosis of syphilis in various periods.

结论梅毒螺旋体特异性IgM抗体检测灵敏度高、特异性强，适用于未经治疗的各个时期的梅毒诊断。

Clinical evaluation of specific IgM treponema pallidum antibody detection

梅毒螺旋体特异性IgM抗体检测的临床评价

An optimized method has been established to simultaneously detect multiple antibodies of HIV, TP and HCV in the serum by the way of microarray.

建立一种用蛋白质微阵列法可同时检测血清中艾滋病毒（HIV），丙型肝炎病毒（HCV）和梅毒螺旋体（TP）抗体的方法。

51 clinical samples were detected by dark-field microscopy and a double-primer PCR, the positive rates were 15.7% and 19.6%, respectively.

检测了51例临床标本，梅毒螺旋体暗视野显微镜检查阳性检出率为15.7%，双引物PCR阳性检出率为19.6%；

Diagnostic Significance of IgM Antibodies to Treponema Pallidum in Cerebrospinal Fluid for Neurosyphilis

梅毒螺旋体IgM抗体对于神经梅毒的诊断意义

Controlled experiments of testing anti-TP and anti-HIV by DIGFA and EIA

免疫胶体金与酶免疫检测HIV抗体和梅毒螺旋体抗体对照实验

Objective To evaluate the significance of IgM treponema pallidum specific antibody in the diagnosis of syphilis in various periods.

目的探讨梅毒螺旋体特异性IgM抗体检测在各个时期梅毒诊断中的意义。

Methods 2557 cases were detected by using ELISA with double antigen sandwich before blood transfusion.

方法：采用免疫ELISA法检测2557例输血前患者血清梅毒螺旋体抗体。

Objective: To establish PCR assay for rapid detection of Treponema pallidum.

目的：建立聚合酶链反应（PCR）用于快速检测梅毒螺旋体。

Conclusions In view of its high specificity and sensitivity, TP-IgM-WB could be used as a confirmatory test for the diagnosis of both symptomatic and asymptomatic neonatal congenital syphilis.

结论梅毒螺旋体IgM抗体蛋白印迹试验可作为一种确诊试验应用于新生儿胎传梅毒，尤其是胎传潜伏梅毒的早期诊断。

Methods: According to specific sequence of Treponema pallidum gene ( tpp47 gene), a pair of specific primers were designed and PCR assay was developed.

方法：根据梅毒螺旋体47ku膜蛋白基因序列，自行设计筛选一对特异寡核苷酸引物，建立梅毒螺旋体的PCR。