The application of sirius red stain and polarization microscopy method in the fibrotic research of hypertrophic scar and keloid

Vibrating mode scanning polarization force microscopy and its applications

振动模式扫描极化力显微镜及其应用

Spin polarization electron microscopy and Monte-Carlo simulation

自旋极化电子显微术及蒙特卡罗模拟

Methods The degrees of fibrosis in normal skin, hypertrophic scar and keloid tissues were detected by Sirius red staining and polarization microscopy method. The SABC immunohistochemistry method and image analysis technique were employed to detect the expression of connective tissue growth factor.

方法分别留取正常皮肤、增生性瘢痕和瘢痕疙瘩组织标本，应用天狼猩红偏振光法检测组织纤维化程度，应用SABC免疫组织化学法和图像分析技术检测组织中结缔组织生长因子的表达。

The electrochemical corrosion behaviors of aluminium and titanium/ alumin-ium dual coatings on uranium surface, in potassium chloride solution with 50 μ g/ g Cl~-, were studied by potentiodynamic polarization and scanning electron microscopy ( SEM).

采用动电位极化技术和扫描电镜（SEM）对U表面Al及Ti/Al双重镀层在含50μg/gCl的KCl溶液中的电化学腐蚀行为进行研究。

The mesophase microstructures in the pyrolyzed residue at successive temperature and time intervals were examined with a polarization microscopy and the mechanism of mesophase transformation was revealed.

用偏光显微镜观察了在不同转化温度和不同停留时间时的中间相显微结构，从中间相显微结构的变化来探讨中间相转化机理。

The influence of Bi~ ( 3+) and tetrabutylammonium bromide ( TBAB) on the dendritic growth behavior of secondary zinc electrode in the alkaline zincate solution was investigated by means of current-time technique, potentiodynamic polarization and scanning electron microscopy.

采用电流-时间曲线、极化曲线和扫描电子显微镜等方法，研究了Bi3+与四丁基溴化铵（TBAB）对可充锌电极在碱性锌酸盐溶液中枝晶生长行为的影响。

The spindle morphological change was observed by immunofluorescence staining and confocal microscopy.

免疫荧光及激光共聚焦显微镜观察MKN45细胞微管改变，流式细胞仪检测MKN45细胞周期及凋亡的变化；

An Improved Immunofluorescence Microscopy for Observing Microtubule Cytoskeleton in Plant Cells

一种改进的观察植物细胞微管的免疫荧光染色方法

Methods The human placental villus-derived trophoblastic cell line ( HPT-8) in vitro was identified and assayed by light microscope, scanning electron microscope, transmission electron microscope, immunohistochemistry and immunofluorescence laser confocal microscopy.

方法通过光镜、扫描电镜、透射电镜、免疫组化和免疫荧光激光共聚焦的方法对体外培养出的HPT-8进行系统鉴定评价。

CD41 specific monoclonal antibody staining was observed by immunofluorescence microscopy.

利用CD41单克隆抗体免疫荧光染色观察培养体系中的细胞情况。

Methods Reverse transcript polymerase chain reaction ( RT PCR), immunofluorescence microscopy and immunohistochemistry methods were used to detect P120 catenin expression in the cells and tissues mentioned above.

方法采用RT-PCR、免疫荧光标记（激光共聚焦显微镜）和免疫组织化学分析，分别对正常肝细胞、肝癌细胞、正常肝组织和肝癌组织中P120ctn进行检测。

The intracellular calcium concentration was measured by laser confocal microscopy. The expressed proteins in the cells were determined by immunofluorescence microscopy and Western blot.

用激光共聚焦显微镜检测细胞内Ca~（2+）的含量，用免疫荧光和western blot法检测细胞内JWA蛋白的分布和表达南京医科大学硕士学位论文水平。

The expression of HN protein was visualized by Western blot and Immunofluorescence microscopy.

用western blot、免疫荧光法检测HN蛋白的表达。

Immunofluorescence double-staining and confocal microscopy were used to examine the configuration of subepithelial immune complex 、 C5b-9 and WT1 around the glomerular basement membrane ( GBM).

运用免疫荧光双套色法，在激光共聚焦荧光显微镜下观察上皮侧免疫复合物沉积构象、C5b-9和WT1在肾小球基膜侧的分布，并对患者肾小球足细胞进行密度定量分析。

AIM: To investigate the effects of fluid shear stress on cytoskeleton of osteoblasts, the authors examined the effects applying confocal laser scanning microscope and Immunofluorescence microscopy.

目的：研究流体剪切力对成骨细胞骨架的影响。

Then tagged with a FLAG Candida antarctica lipase B protein with three kinds of C-terminal N-anchored fusion protein and used immunofluorescence microscopy, that the newly constructed yeast display common carrier is successful.

再以带有FLAG标签的南极假丝酵母脂肪酶B蛋白的C末端同3种锚定蛋白N端融合，并利用免疫荧光显微镜检测，证明新构建的毕赤酵母展示通用载体是成功的。

Immunofluorescence laser confocal microscopy was use to observed these cells after crawled on the slide.

细胞爬片，免疫荧光激光共聚焦显微镜观察。

The expression of α 3 and/ or α 5 subunits of nicotinic acetylcholine receptor in rat pituitary: double immunofluorescence microscopy study

烟碱型乙酰胆碱受体α3和/或α5型在大鼠脑垂体中的表达：荧光双标免疫组织化学研究

Methods After anoxia/ oxidative stress, immunofluorescence staining and confocal microscopy were used to study the distribution of integrin α 3 on the membrane of TECs under 2 experimental conditions, i.e., fixed or cultured living cells.

方法采用免疫荧光激光共聚焦观察乙醇固定及培养活细胞两种实验条件下TEC整合素α3分布的染色结果。

The intracellular transport capabilities of these peptides were tested with indirect immunofluorescence assay and confocal microscopy.

采用间接免疫荧光与激光共聚焦显微镜技术，分别对上述18肽和9肽的穿膜能力进行了动态观察。