Methods Bladder transitional cells were obtained from frozen bladder membrane sections and BTCC cells from frozen BTCC tissue sections by using LCM. RNA was extracted and about 120 ng RNA was linearly amplified in vitro, then 20 ug aRNA was obtained.

  • 方法采用LCM技术分别从正常膀胱黏膜及膀胱癌组织冰冻切片中获取膀胱移行上皮细胞及癌细胞,提取RNA,并对120ng上皮细胞RNA进行线性扩增,获得aRNA20μg。

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