pcr的例句大全

Hematopoiesis regulation factors such as SCF, IL-3 , TNF-α and IFN-� � mRNA semi-quantitative expression were observed by RT-PCR .

通过RT -PCR 法观察其骨髓造血细胞调控因子干细胞因子(SCF)、白细胞介素3(IL -3 ) 、 肿瘤坏死因子α(TNF - α)、干扰素γ(IFN - γ)mRNA的 半定量 表达情况.

Methods Aggrecan IGD cDNA was amplified by RT-PCR and inserted into pUCm-T vector .

方法　取人软骨细胞,逆转录多聚酶链反应(RTPCR)扩增aggrecan球 间区域 ( IGD )片段.

PPAR � � expression of GH 3, MMQ and ATt 20 cell line was evaluated by RT-PCR and Western Blot.

RT -PCR 及WesternBlot方法检测PPARγ在GH3 、 MMQ、ATt20等垂体瘤细胞株中的表达情况.

PUC 18 transferring and ERIC-PCR showed that the recombination strain SR-15 could grow in the plant stably.

SR-15 菌株通过质粒PUC-18转化和ERIC -PCR 再分离实验验证,结果显示重组菌株在植株体内稳定定位,具有稳定的内生特性.

Expression of TGF � � 1 was evaluated by RT PCR technique.

逆转录聚合酶链反应(RT?PCR)方法测定肺组织内TGF?β1的表达.

The shoot meristems were transformed. But no plants were positive, according to PCR results. 6.

对茎尖进行了转化，只获得抗性植株，但未检测到PCR阳性植株。

Studies on Cultivar Classification of Osmanthus fragrans by ISSR-PCR Analysis

ISSR-PCR技术在桂花品种分类研究中的应用

We examined 112 samples collected on the filter paper disks in the Solomon islands to compare the value of polymerase chain reaction ( PCR) and microtiter plate hybridization ( MPH) methods with conventional microscopic examination for detection of malaria.

应用聚合酶链反应（PCR）及微滴度板杂交试验（MPH）对来自高疟疾流行区、收集于滤纸片的112例干血滴样品进行检测，并对同一人群作镜检进行比较。

The procollagen gene expression was analyzed RT-PCR.

PCR用于分析前胶原基因表达.

Methods Sixty-two saliva samples collected from 62 children were detected HP with nested PCR.

方法采用巢式聚合酶链式反应对62例儿童的唾液进行HP检测。

Eighty-three strains of Newcastle disease virus ( NDV) were isolated from cases during epidemic in different parts of China in 2005.The main functional region of the F gene were amplified by RT-PCR and sequenced.

采用2005年从国内不同地区、不同宿主分离到的NDV分离株，选取其中具有代表性的83株，用RT-PCR技术扩增其F基因重要的功能区片段，进一步进行序列测定。

The Applied Research on the RT-PCR Nucleic Acid Detection and ELISA Antigen Detection Kit of CSFV

猪瘟病毒RT-PCR核酸检测与ELISA抗原检测试验应用研究

Establishment of RT-PCR Assay on CSFV E2 Gene and Study on CSFV Molecular Epidemiology

猪瘟病毒E2基因RT-PCR检测方法的建立及病毒分子流行病学研究

Detection of EHEC o157: h7 by using complex probe real-time fluorescent PCR

复合探针实时荧光PCR检测大肠杆菌O157：H7

Methods ICE mRNA expressions after cortex repetitive ischemia-reperfusion ( IR) were determined by RT-PCR in VD mice.

方法利用血管性痴呆模型，RT PCR技术检测全脑重复缺血再灌（IR）后ICE mRNA的表达。

Quick Detection of Citrus exocortis viroid ( CEVd) by One-step RT-PCR

利用一步法RT-PCR检测柑橘裂皮病类病毒

Preliminary study on One-step RT-PCR detection of Citrus exocortis viroid ( CEVd) and its distribution in different parts of sweet orange

柑橘裂皮病类病毒的一步RT-PCR法检测及其在甜橙体内的分布

Simultaneous detection of Citrus tristeza virus ( CTV), Citrus exocortis viroid ( CEVd) and Citrus tatter leaf virus ( CTLV) by multiplex RT-PCR

柑橘衰退病、裂皮病和碎叶病的多重RT-PCR检测方法研究

Preliminary study on comparison of Fast Micro-extraction method and SDS-KAc method, detecting CEVd in Etrog citron with symptoms by One-step RT-PCR, revealed that SDS-KAc method was preferable for eliminating non-specific band.

分别采用快速微量核酸提取法和SDS-KAc抽提法在表现症状的Etrog香橼中提取核酸，采用一步RT-PCR法对CEVd进行检测，结果显示SDS-KAc法可以消除带毒样品中非特异性条带。

Four microsatellite markers linked to FecB locus on 6 chromosome in ovine were analyzed for 108 animals by PCR and denaturing polyacrylamide gel electrophoresis.

选择4个位于绵羊6号染色体上且与FecB基因紧密连锁的微卫星标记，对小尾寒羊基因组进行PCR扩增后，采用最小二乘法估计各等位基因片段对产羔数的影响。