pcr的例句大全

Semi quantitative RT-PCR analysis show that there were notable difference between different lines. Expression of gene driven by CatB promoter in root is more than in leaf. Expression level of the latter was extremely weak.

半定量RT-PCR结果显示，不同株系间的表达量有明显差异，而且由水稻CatB启动子驱动的外源基因在根中的表达量远远大于在叶子中的表达量，后者只有微弱的表达。

By using RT-PCR, a study was carried out on the time and spacial order of the diapause hormone gene expression in oriental tobacco budworm on the transcription level.

利用RT PCR法，在转录水平上对烟夜蛾滞育激素（diapause hormone，DH）基因的时空表达进行了研究。

Cholesterol ester transfer protein ( CETP) gene polymorphism was assayed by PCR-RFLP.

以PCR RFLP检测胆固醇酯转运蛋白（CETP）基因TagⅠB多态性。

In this study, the 28-day-old broilers from Exp.1 were used as the test materials after slaughtering, and the same segment of jejunum was removed from each chick ( four chicks per treatment) and was prepared for the BBMV and real time PCR.

本试验以试验一中肉仔鸡为试验材料，28日龄时，每个重复取4只鸡，屠宰后取每只鸡相同部位空肠肠段，制备BBMV及用作实时定量检测。

Results: A total of 4 alleles with 5~ 8 repeat units were observed and the genetic diversity was estimated to be 0.501 3. Decomposed and keratinized samples were typed successfully by using the methods of Chelex-100 with DTT and PCR buffer, respectively.

结果河南汉族214例无关个体中检出4种等位基因，重复5~8次，基因差异度0.5013。腐败肌肉及角化组织用DTT联合Chelex-100法和PCR缓冲液法均分型成功。

A Study on PCR to Detect Streptomycin and Kanamycin Resistant Gene aadA and aadB of Swine Salmonella

PCR技术检测猪源沙门氏菌链霉素、卡那霉素耐药基因（aadA、aadB）的研究

Under conditions of 27 t, pH 8.0 and salinity 33, we studied the method of latent infection of white spot syndrome virus ( WSSV) in Penaeus japonicus by nested-PCR and dot blot hybridization.

在27℃，pH8.0，盐度33条件下，用套式PCR方法和斑点杂交方法对日本对虾（Penaeus japonicus）体内潜伏性感染WSSV的方法进行了研究。

By designing primers in the conservative coding sequences flanking a polymorphic site, it is possible to exploit a PCR-based marker called amplified consensus genetic marker ( ACGM), which is transferable among different species.

通过在多态位点两侧的保守编码序列上设计引物，可以开发出在不同物种间通用的基于PCR的分子标记，称为扩增共有序列遗传标记（ACGM）。

The detection rate of the three techniques ( Pathogenicity test, DIA and PCR assay) for Xac reach 100% for symptomatic citrus sample.

PCR、DIA和致病性测定法检测田间显症样品的检出率均可达到100%，而检测柑桔无症样品的检出率依次降低。

The full-length of THRSP α gene of ducks cloned by the author was used to detect the polymorphism in THRSP α intron. The association of mutation locus with growth and body compositions of 238 Pekin ducks at 6 wks was investigated by PCR-RFLP method.

根据克隆出的鸭THRSPα基因全长序列，采用PCR-RFLP法检测THRSPα内含子多态与238只6周龄北京鸭生长、屠体性状的关系。

Weight gain ratio ( WGR), special growth rate ( SGR), feed efficiency ( FE) and, protein conversion efficiency ( PCR) of fish were improved by the elevation of dietary phosphorus level and reached maximal value at 1.32% total phosphorus.

随着饲料中磷添加水平的提高，黑鲷幼鱼的增重率、特定生长率、饲料效率和蛋白质效率呈上升趋势，当饲料总磷含量为1.32%时，各项指标均达到最高水平。

The exon 28 of authenticity vWF gene was studied by PCR, denaturing gradient gel electrophoresis ( DGGE) and sequencing in the type 2A vWD.

对2A型血管性血友病的患者用多聚酶链反应、变性梯度凝胶电泳，结合测序的方法进行了基因突变的研究。

Observation on Dynamic Change in Photo inactivation Damaged Nucleic Acid of VSV by RT-PCR Method

RT-PCR法观察光化学法损伤VSV核酸的动态变化

Isolation of VSG gene of Trypanosoma evansi by RT-PCR

RT-PCR法分离伊氏锥虫VSG基因的研究

Polymerase chain reaction-PCR technique was used to obtain Vitreoscilla hemoglobin gene ( vgb) using its genomic DNA as a template.

以透明颤菌染色体基因组DNA为模板，利用PCR技术获取透明颤菌（Vitreoscilla）血红蛋白基因（vgb）。

Detection of TDH Gene of Vibrio Parahaemolyticus in Diarrheal Stool Using Rapid PCR

聚合酶链反应检测粪便中的副溶血性弧菌的TDH基因

Genome DNA was extracted by TKM method. Exon 10 of TSHR gene sequence was amplified, PCR production was detected by direct and reverse sequencing, and the results and human TSHR gene sequence Genbank were compared.

用TKM法提取基因组DNA，PCR扩增TSHR基因第10外显子序列，对PCR产物正反向测序，测序结果同Genbank人TSHR基因序列对比。

Further, Touchdown PCR with gene-specific primer also proved the existence of RHL gene with same length in a same region in corn and barley genome.

但由于基因组大小不同、基因密度不一致以及微共线性保守性程度低，其在各自染色体位置上存在偏差。

Methods Compare 198 clinic specimen results detected by RLB and mycoplasmas specific species PCR.

方法应用支原体反向线点杂交方法和种特异性PCR方法同时检测198例临床标本，比较两种方法的检测结果。

For reducing the experiment cost, the primary RT-PCR methods of PVX and PVY were improved.

为了降低实验成本，对已建立的PVY、PVX两种病毒的RT-PCR检测体系进行改进。