polymerase的例句大全

Detection of pathogenic Entamoeba histolytica DNA in liver abscess aspirates with polymerase chain reaction

用聚合酶链反应检测肝脓液中溶组织内阿米巴复制基因片段

Method We detected ACE gene polymorphism and the association between ACE gene polymorphism with urinary protein excreting weight, hematuria, hypertension, azotemia using polymerase chain reaction in 186 children who suffered persistent proteinuria.

方法用PCR方法检测了186例儿童持续性蛋白尿患儿ACE基因多态性及ACE基因多态性与尿蛋白量、血尿、高血压、氮脂血症的关系。

PCR ( polymerase chain reaction) technology is specific, sensitive, simple and rapid. Research on detecting pathogenic bacteria in food by PCR technology is ascendent.

PCR（聚合酶链式反应）技术具有特异性强、灵敏度高、操作简单、快速的特点，使用PCR技术检测食品中致病菌的研究方兴未艾。

Methods Reverse transcript polymerase chain reaction ( RT PCR), immunofluorescence microscopy and immunohistochemistry methods were used to detect P120 catenin expression in the cells and tissues mentioned above.

方法采用RT-PCR、免疫荧光标记（激光共聚焦显微镜）和免疫组织化学分析，分别对正常肝细胞、肝癌细胞、正常肝组织和肝癌组织中P120ctn进行检测。

Methods: The circled TA plasmid was digested and linearized with restriction endonuclease. The ends of the plasmid were repaired and made blunt with Klenow Fragment. A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase.

方法：将环化质粒以限制性内切酶消化成线性后，以Klenow大片段将末端补平，再用taq DNA聚合酶在线性载体的两个5′末端各加上一个非配对的T。

Taq DNA polymerase is a kind of the highly heat-stable DNA polymerase, that was isolated from thermophilic bacterium Thermus aquaticus. Because of its high thermostability, high specificity, sensitivity and other excellent features, Taq DNA polymerase is widely used in the PCR amplification.

taq DNA聚合酶是一种来源于嗜热菌Thermus aquaticus的高度热稳定的DNA聚合酶，并以其耐高温、较高的特异性、灵敏度等优良特性被广泛应用在PCR扩增中。

Although the commercial production of Taq DNA polymerase has come true for many years and a lot of technique improvement has been made, the activity ratio of the enzyme preparation is not high, some methods of procedure too complicated, or the production cost is high.

虽然taq DNA聚合酶已经商品化生产多年，制备方法较多，但是目前一些方法生产的酶制剂比活性不高，而一些方法又过于繁琐，增加生产成本。

Study on role of DNA polymerase β in alkylating agent MNNG induced untargeted mutagenesis

DNA聚合酶β在烷化剂MNNG引起的非定标性突变中的作用

Concentration of primer, magnesium chloride, dNTP, template DNA, Taq DNA polymerase and annealing temperature in PCR and the quantity of PCR product and endonuclease and digesting time in digestion process affect the profiles of the whole experiment.

PCR反应体系中不同模板含量、引物浓度、taq DNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。

In order to establish the optimized SSR detection system, the concentrations of DNA, Mg~ ( 2+), Taq DNA polymerase, dNTPs, and the annealing temperature were optimized for SSR-PCR reaction.

为建立适宜的SSR反应体系，对影响SSR-PCR扩增的模板DNA浓度、Mg~（2+）的用量、taq DNA聚合酶浓度、dNTPs浓度、退火温度等因素进行了优化。

The DNA repair techniques with Taq DNA polymerase can greatly enhance the success rate of STR genotyping for high degrade samples, which is suitable for the STR genotyping of high degrade samples. 3.

使用taq DNA聚合酶修复技术可以极大地提高高度降解检材STR分型的成功率，适合高度降解检材STR分型的应用。

Methods Slides of metaphase chromosome were examined by the method of PCR in situ. And PCR reactions without Taq DNA polymerase, primer and bio 11 dUTP were set up as control groups.

方法应用原位PCR的方法对豫医无毛小鼠中期染色体标本的无毛基因进行检测，并设立PCR反应液中无taq DNA聚合酶、无引物、无bio-11-dUTP等进行多组对照。

[ METHOD] Different levels on concentration of DNA template, primer, dNTP mixture and Taq DNA polymerase and annealing temperature were set in this experimentation. All factor influence for SRAP-PCR in peanut genome was investigated.

【方法】对模板DNA、引物、dNTP mixture、taq DNA聚合酶浓度及退火温度设置不同梯度，研究各因素对PCR结果的影响；

One of the mechanisms causing gene mutation might be to change of the Taq DNA polymerase conformation. It interfers with the enzyme-substrate-dNTP binding site and further causes replication mismatch.

这一实验结果也说明了稀土是一种诱变剂，引起基因突变的机制之一可能是改变taq DNA聚合酶的立体构象，干扰了酶与底物dNTP结合位点，从而引起复制错配。

High content of protein, phenol, tannin, pigment and polysaccharide in plant tissues usually affect the activity of Taq DNA polymerase and Lead to failure of PCR reaction.

植物体内所含的蛋白质、单宁、酚类、多糖及色素等次生代谢物质影响taq DNA聚合酶的活性，是导致PCR扩增反应失败的主要因素。

Moreover, the concentrations of Mg2+, dNTP and Taq DNA polymerase in allele-specific PCR were higher than that in conventional PCR.

在等位基因特异PCR中，Mg2+、dNTP及taq DNA聚合酶的用量均大于普通PCR。

Taq DNA polymerase is an ideal enzyme for DNA sequencing because it's fast, highly processive and active over a broad range of temperature.

taq DNA聚合酶具有反应速度快、温度作用范围广及良好的续进性等特点，可视为一种理想的DNA顺序分析酶。