To optimize the multiplex RT-PCR, the concentration of primers and annealing temperature were repeatedly tried. Final results showed that a multiplex RT-PCR system to simultaneously detect HLB, CEVd and CTV was firstly established successfully.

  • 就影响多重PCR的主要因素引物浓度和退火温度进行了一系列优化,建立了能同时检测HLB、CEVd、CTV的多重RT-PCR技术体系。

  • 互联网摘选 2025-01-20 17:19:02

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