From Antisense to Antigene: an New Breach of Antigene Strategy

The Fusion Expression of Green Fluorescent Protein and HCV Core Antigene in Escherichia coli Cells

绿色荧光蛋白与HCV核心蛋白的融合及在大肠杆菌中的高效表达

In addition, the unique chemical, physical and biological properties of PNAs have been exploited to produce powerful biomolecular tools, antisense and antigene agents, molecular probes and biosensors.

此外，鉴于肽核酸独特的理化和生物学性能，人们正尝试将其用于生物分子工具、反义和反基因制剂、分子探针和生物传感器。

Objective To provide experimental basis for antigene radiation therapy through exploring the effects of antigene strategy on androgen receptor ( AR) expression and proliferation of prostate cancer cells.

目的探讨反基因策略对雄激素受体（AR）表达和前列腺癌细胞增殖的影响。

Methods DAT positive samples can be divided into AIHA group and non AIHA group, process immunity types, and test the samples of IgG or IgG+ C 3 with elution, use elution liquid and standard antibody screen cell to process indirect antiglobulin test ( IAT).

方法DAT阳性标本分AIHA组和非AIHA组，进行免疫分型，然后把IgG或IgG+C3的标本进行放散试验，利用放散液与标准抗体筛查细胞进行间接抗人球蛋白试验（IAT）。

The results showed that out of 38 patients with AIHA, 11 cases ( 31.6%) of ABO blood group were difficultly typed, indirect antiglobulin test were positive, and contradiction between cells typing and sera typing were observed.

结果表明：38例AIHA患者中，ABO血型难定者11例（31.6%），均为间接抗球蛋白试验阳性及正反定型不合。

The Highest titer of anti D ( IgG) measured by GAT was 1 ∶ 256, and the sensitivity of GAT was higher than that of traditional antiglobulin test, and approximately equal to manual polybrene test.

GAT可测的抗D（IgG）最高效价为1∶256，反应灵敏度明显高于试管法，而近似于聚凝胺法。

Methods The antibody specificity of two patients' sera reacted to twelve panels of reagent red blood cells and 4 blood group antigens known their blood types was analyzed and identified in saline medium and low ionic indirect antiglobulin medium.

方法对2例患者的血清，与已知血型的试剂红细胞和4个已知Mur抗原，在盐水介质、低离子间接抗球蛋白介质，分析鉴定出其抗体的特异性。

Methods The blood samples of 23 patients with AIHA were identified by routine typing and typing after chloroquine elution test on DIANA auto instrument. Direct and indirect antiglobulin test were done in the same time.

方法用DIANA全自动血型仪对23例AIHA患者进行ABO、Rh（D）血型常规定型及氯喹放散实验后定型，同时进行直、间接抗人球蛋白试验。

The authors propose that the appearance of HPB correlates chiefly with the following factors: such as, the level of umbilical bilirubin, neonatal blood group, and the result of modified direct antiglobulin test.

作者认为，生后24小时发生高胆由多种因素所致，与脐血胆红素值、于血症、改良直接抗人球蛋白试验关系较大。

The positive rates of direct antiglobulin test ( DAT), elution test and indirect antiglobulin test ( IAT) were 39.2%, 88% and 69.6% respectively.

三项试验中直接抗人球蛋白试验阳性49例（39.2%），释放试验阳性110例（88%），游离试验阳性87例（69.6%）；

Methods Semen of 303 cases of male infertility were examined for UU by cultivation and AsAb in semen were simultaneously detected by mixed antiglobulin reaction ( MAR).

方法收集男性不育症患者303例，采用计算机辅助分析系统行精液常规分析，用UU分离鉴定培养液检测精液UU，采用混合抗球蛋白反应试验（mixed antiglobulin reaction，MAR）检测精液AsAb。

Result: Using the routine blood group and serology techniques, autoantibody interfered with ABO typing in 10 cases, most of whom were indirect antiglobulin test positive.

结果：28例中10例ABO定型受干扰，多为间接抗球蛋白试验阳性者及正反定型不合者。

Objective To distinguish types of the direct antiglobulin test ( DAT) positive, help clinical to diagnose exactly the Autoimmune Hemolytic Anemia ( AIHA);

目的通过直接抗人球蛋白试验（DAT）阳性的类型鉴别，有助于临床对自身免疫性溶血性贫血（AIHA）的正确诊断；

All direct antiglobulin test were positive and higher frequency of the positive results in in direct antiglobulin test appeared in the cases those ABO blood group difficultly typed.

23例直抗均为阳性，间抗阳性更易出现在ABO血型正反定不符病例中。

Micro-colume gel immuno-assay in the direct antiglobulin test for hemolytic disease of the newborn

微柱凝胶技术对新生儿溶血病直接抗人球蛋白的检测效果

METHODS: Antiglobulin test ( AGT) of drug simulated in vitro were performed with hemo immunology.

方法：作血液免疫学体外药物模拟抗球蛋白试验（AGT）。

A modified~ ( 125) i-labeled antiglobulin technique

一种固相~（125）I标记抗球蛋白的技术

The causes of transfusion reaction was detected by antiglobulin test of Coombs.

通过血型血清学检测来寻找输血反应原因。

Experimental evaluation of the gel antiglobulin test

凝胶抗球蛋白试验的特点及实验分析

Distinguish types and clinical significance of direct antiglobulin test positive

直接抗人球蛋白试验阳性的类型鉴别及临床意义