42K envelope protein gene of Contagious Ecthyma virus were amplified by specification primers and recombinant to different expression vector. The 9 strains stable recombinant were screened by dot blotting and southern blotting with DNA probe labeled DIG.

  • 用特异性引物扩增羊传染性脓疱病毒42K囊膜蛋白基因,与不同的表达载体重组后用DIG标记的特异性DNA探针进行Dot-blotting和southern blotting杂交检测,挑选出能稳定传代的重组菌9株。

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