new melodies bresk forth from the heart;

"Waking Life" gives audiences in Venice a bresk from the tales of a young man unable to wake up from a series of connecting dreams.

《半》给威尼斯的观众们描述了一个年轻人无法从一系列相关联的梦中醒来。

Well, let us get away from that. Has Sven anything to offer as a manager?

好吧, 别提这些恶心事了. 让我们来说说,埃里克森的教练才能 怎么样 ?

Sven Goran Eriksson has agreed to take charge of Manchester City, according to Sky Sports News.

根据天空体育新闻报道,斯文埃里克森已经同意执教曼城俱乐部.

Sven swam back again.

塞文游了回来.

Finally, I am sorry that Sven-Goran Eriksson is leaving the job as England manager.

最后, 令我遗憾的是艾里克森将离开英格兰主教练的位置.

Sven has obviously done his homework as far as his personality is concerned-he'll fit in.

斯文很明显像他的个性一样做好了功课 —— 他能适应.

England Sven Goran Eriksson has admitted he is worried about Frank Lampard's form.

英格兰队艾里克森承认,他为兰帕德的状态感到担忧.

'sven thinks it might be best for the winter, anyhow. "

敏妮赶快不失时机地说: “ 史文觉得冬天还是回去的好.

It will form part of Sven-Goran Eriksson's preparation for next year's World Cup in Germany.

这将是斯文 - 格兰.埃里克森为明年德国世界杯准备的系列赛之一.

Ahead of that, Sven will name his provisional squad on May 8.

在此之前, 斯文会在5月8号确定最终的名单.

Sven visited the Hospital, where he is a patron, and divided his time between three wards.

斯文拜访了医院的3间病房, 他是那家医院的资助人.

Sven swam over the sea.

塞文在海上游泳.

Methods: The circled TA plasmid was digested and linearized with restriction endonuclease. The ends of the plasmid were repaired and made blunt with Klenow Fragment. A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase.

方法：将环化质粒以限制性内切酶消化成线性后，以Klenow大片段将末端补平，再用taq DNA聚合酶在线性载体的两个5′末端各加上一个非配对的T。

Taq DNA polymerase is a kind of the highly heat-stable DNA polymerase, that was isolated from thermophilic bacterium Thermus aquaticus. Because of its high thermostability, high specificity, sensitivity and other excellent features, Taq DNA polymerase is widely used in the PCR amplification.

taq DNA聚合酶是一种来源于嗜热菌Thermus aquaticus的高度热稳定的DNA聚合酶，并以其耐高温、较高的特异性、灵敏度等优良特性被广泛应用在PCR扩增中。

With genome DNA as template, three upstream primers ( R1 、 R2 、 R3) targeting point mutation of K-ras oncogene codon 12 and common downstream primer ( R4) were added respectively. PCR was employed to amplify corresponding fragment with Taq DNA polymerase.

以基因组DNA为模板，分别加入针对K-ras第12密码子的三种主要突变方式设计的PCR上游引物R1、R2、R3和共同的下游引物R4，在taq DNA聚合酶作用下扩增相应片段。

Although the commercial production of Taq DNA polymerase has come true for many years and a lot of technique improvement has been made, the activity ratio of the enzyme preparation is not high, some methods of procedure too complicated, or the production cost is high.

虽然taq DNA聚合酶已经商品化生产多年，制备方法较多，但是目前一些方法生产的酶制剂比活性不高，而一些方法又过于繁琐，增加生产成本。

After Taq DNA polymerase had been isolated and purified from the thermophilic bacteria ( Thermus aquatics) in aquatic habitat by Randall K. Saiki and his colleagues in 1988, it was widely used in PCR techniques.

1988年，Randall K.Saiki等[1.2]从水生栖热菌（Thermus aquatics）中分离纯化到了taq DNA聚合酶，之后它广泛应用于PCR技术。

Concentration of primer, magnesium chloride, dNTP, template DNA, Taq DNA polymerase and annealing temperature in PCR and the quantity of PCR product and endonuclease and digesting time in digestion process affect the profiles of the whole experiment.

PCR反应体系中不同模板含量、引物浓度、taq DNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。

In order to establish the optimized SSR detection system, the concentrations of DNA, Mg~ ( 2+), Taq DNA polymerase, dNTPs, and the annealing temperature were optimized for SSR-PCR reaction.

为建立适宜的SSR反应体系，对影响SSR-PCR扩增的模板DNA浓度、Mg~（2+）的用量、taq DNA聚合酶浓度、dNTPs浓度、退火温度等因素进行了优化。

The DNA repair techniques with Taq DNA polymerase can greatly enhance the success rate of STR genotyping for high degrade samples, which is suitable for the STR genotyping of high degrade samples. 3.

使用taq DNA聚合酶修复技术可以极大地提高高度降解检材STR分型的成功率，适合高度降解检材STR分型的应用。